BIOL 202 Lecture Notes - Lecture 17: Fragile X Syndrome, Intellectual Disability, Convulsion
25 Feb 2013
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Document Summary
A probe is a complementary sequence that sticks onto the restriction sites to show where the cuts were made. Restriction sites could be added and removed due to mutations. E. g. a deletion that removes a site that decreases the sequence length, or an insertion that increases the frequency length. Triangular lines indicate identical twins while bent lines indicate non-identical twins. The disease creates a large sized fragment when compared to normal ones, when the ta is flipped into at. Rflp is usually found through trial and error, takes too long to locate new sites. They are also di-allelic, not very useful for a large population. Rflp analysis also requires huge amounts of dna. The analysis process is also time consuming (isolation, digestion, electrophoresis and blotting). Pcr amplifies the amount of dna present by a huge amount. Pcr primers are on the two sides of the dna segment and are outside of the region that would be amplified.
