Your friend is trying to amplify a DNA sequence using PCR, but he didnât feel too confident about designing the primers, so he ordered two different sets of primers (4 primers total). Unfortunately, neither set produced a visible product when ran on a gel through electrophoresis. Below are the primers that he used and the DNA to be amplified:
DNA template:
5â ATTCGGACTTG----(750 bases to amplify)----GTCCAGCTAGAGG 3â
3â TAAGCCTGAAC--------------------------------------CAGGTCGATCTCC 5â
Primer pair #1 (a) 5â GGACTTG 3â (b) 5â GTCCAGC 3â
Primer pair #2 (a) 5â TTCAGGC 3â (b) 5â AGCTGGA 3â
a) With the DNA denatured as below, show where primer pair #1 should anneal. Would this end up in a PCR product, why or why not?
5â ATTCGGACTTG----(750 bases to amplify)----GTCCAGCTAGAGG 3â
3â TAAGCCTGAAC--------------------------------------CAGGTCGATCTCC 5â
b) With the DNA denatured as below, show where primer pair #2 should anneal. Would this end up in a PCR product, why or why not?
5â ATTCGGACTTG----(750 bases to amplify)----GTCCAGCTAGAGG 3â
3â TAAGCCTGAAC--------------------------------------CAGGTCGATCTCC 5â