Chemical Laboratory Technician BIC472 Lecture Notes - Alkaline Phosphatase, Reaction Rate
21 Mar 2013
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You are required to plot your data to determine some of the properties of calf- intestinal phosphatase. Include tables of your raw data and calculated values, and show one sample of each calculation performed. The plots required and the information to be obtained are summarized below: Calculate the reaction velocity (nmol/min. ) in each tube. [p] = 0. 000013587mol nmol p= 0. 000013587mol x 10 9nmol/mol x 0. 0065l nmol p= 88. 3. In a sentence, state what is/are the optimal temperature(s) for alkaline. 3. phosphatase activity: the optimal temperature for alkaline phosphatase would be 35 c. This is due to the fact that at an incubation period of 35 c for 15minutes produced the highest absorbance reading, which means more product was formed from the reaction. C1 v1 = c2 v2 (1mm)(0. 25ml) = c2 (3ml) [p] = 0. 000001739mol nmol p= 0. 000001739mol x 10 9nmol/mol x 0. 0065l nmol p= 11. 30. Perform two plots: a michaelis-menten plot and a lineweaver-burk plot.
