BIOL 303 Lecture Notes - Lecture 27: Taq Polymerase, Sanger Sequencing, Polymerase Chain Reaction
14 Apr 2017
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Uses dna polymerase to rapidly replicate a specific dna sequence in a test tube. 5: extend, repeat, repeat again, 9 and again. Denature dna with heat - 92 degrees, 30 . Anneal primers (short dna fragments 10-30 nucleotides long) - 50 degrees, 1". Have a million copies of the region between primers. Produces over a million copies and separate them out. Check the accuracy of a selected sequenced area of a genome. Deduces a dna sequence by aligning pieces that differ from each other by the end base: Variations label, cut, and/or immobilize the dna pieces in different ways, greatly speeding the process. Immobilizes dna pieces on different types of materials, to read and overlap millions of pieces at once. First human genome was in 2000, and took 8 years and costs billion, now ,000. Millions of fragments in a grid, laser reads each one after ever nucleotide addition.
