BIOL 200 Lecture Notes - Lecture 14: Reaction Rate, Processivity, Titration

• School: McGill University
• Department: Biology (Sci)
• Course: BIOL 200
• Professor: Mathieu Roy

Document Summary

Amounts of enzymes can either be expressed as molar amounts, as with any other chemical, or measured in terms of activity, in enzyme units. enzyme activity = moles of substrate converted per unit time = rate reaction volume. Enzyme activity is a measure of the quantity of active enzyme present and is thus dependent on conditions, which should be specified. Enzyme activity as given in katal generally refers to that of the assumed natural target substrate of the enzyme. Enzyme activity can also be given as that of certain standardized substrates, such as gelatin, then measured in gelatin digesting units (gdu), or milk proteins, then measured inmilk clotting units (mcu). The units gdu and mcu are based on how fast one gram of the enzyme will digest gelatin or milk proteins, respectively. The specific activity of an enzyme is another common unit. This is the activity of an enzyme per milligram of total protein (expressed in mol min 1mg 1).