🏷️ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION →
Log in
HomeStudy Guides420,000CA160,000

Biology 1002B Study Guide - Final Guide: Programmed Cell Death, Antitoxin, Missense Mutation

62 views7 pages
pearbuffalo731
18 Apr 2017
School
Western University
Department
Biology
Course
Biology 1002B
Professor
Tom Haffie

For unlimited access to Study Guides, a Grade+ subscription is required.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers

Related Documents

Biology 1002B Chapter Notes - Chapter Outcomes: Blastoderm, Start Codon, Missense Mutation
erinimpala672
Biology 1002B
Final Exam
Study Guide
Biology 1002B Study Guide - Final Guide: Start Codon, Blastoderm, Missense Mutation
chocolategoldfish786
Biology 1002B Lecture Notes - Lecture 8: Chromatin, S Phase, Biochemical Cascade
copperwasp664

Related Questions

Part B

Think about the DNA coding sequence of a gene. If an A were swapped for a T, what kind of mutation could it cause and why?

a) It could cause a frameshift nonsense or frameshift missense mutation because it would change the reading frame of the codon triplet.
b) It could cause a silent, missense, or nonsense mutation because those are the types that can be caused by a nucleotide-pair substitution like this one.
c) It could cause a nonsense mutation because the sequence would no longer be the same, so the protein would be shorter and non-functional.

d) It could cause a silent mutation because A and T are complementary to each other so it is not really a substitution mutation.

Part C

Why is a frameshift missense mutation more likely to have a severe effect on phenotype than a nucleotide-pair substitution missense mutation in the same protein?

a) A substitution missense mutation causes the protein to be shorter and thus non-functional.
b) A frameshift missense will cause the codons to be out of order, but a substitution missense does not change the order of the codons.
c) A frameshift missense mutation will cause an early Stop codon, but a substitution missense might be silent.

d) A substitution missense affects only one codon, but a frameshift missense affects all codons downstream of the frameshift.

Part D

Which of the following sequences shows a frameshift mutation compared to the wild-type mRNA sequence?

a) wild-type 5’-AUGCAUACAUUGGAGUGA-3’
mutant 5’-AUGCAUACAGAGUGA-3’
b) wild-type 5’-AUGCAUACAUUGGAGUGA-3’
mutant 5’-AUGCAUACGUUGGAGUGA-3’
c) wild-type 5’-AUGCAUACAUUGGAGUGA-3’
mutant 5’-AUGCAUACAUCUGGAGUGA-3’
d) wild-type 5’-AUGCAUACAUUGGAGUGA-3’
mutant 5’-AUGCAUGUGACAUUGGAGUGA-3’

Part F

Suppose that the triplet of nucleotides indicated in bold (AGC) spans two codons, that is, CTA and GCC. If the triplet AGC were deleted from this DNA coding sequence, what effect would it have on the resulting protein?

5’-ATGCTAGCCTATCGTAAC-3’

a) The two flanking codons would be altered, but the rest of the amino acid sequence would be the same because there would be no frameshift.
b) The entire amino acid sequence would be altered due to the frameshift.
c) All of the amino acids after the deletion would be altered due to the frameshift.
d) All of the amino acids up to the deletion would be altered due to the frameshift.

1. Gennie is wild type. Below is a segment of her DNA. The topstrand is the coding region, and the reading frame begins with thefirst codon that codes for met (start codon).

5' GCATGTTCTTGT 3'

3' CGTACAAGAACA 5'


Kevin is mutant. Below is a segment of his DNA. The top strandis the coding region, and the reading frame begins with the firstcodon that codes for met (start codon).

5' GCATGTTTTTGT 3'

3' CGTACAATAACA 5'


What type of mutation does Kevin have?

A) silent

B) misssense

C) nonsense

D) frameshift


2) A type of dwarfism results from the production of mutantSHR-1 protein. You look at the length of the mutant SHR-1 and thenormal (wild-type) SHR-1 protein, and discover that the mutantSHR-1 protein has fewer amino acids.


What do you expect to find when you examine the DNAsequence?

A) nucleotides were deleted

B) additional nucleotides were added

C) one nucleotide was changed

D) without additional information, any of the above arepossible.


3) Below are two DNA coding strand sequences. The 5' ATG encodesthe in-frame strat codon.

Tom's DNA sequence is:

5' ATG CGC TTA CCC TTA CTC CTA TAA 3'


Harry's DNA sequence is:

5' ATG CGC TAA CCC TTA CTC CTA TAA 3'


Harry's mutation causes the premature termination of:

A) Replication

B) Transcription

C) Translation

D) Answer B and C

E) All of the above


2) For the hypothetical DNA sequence/gene below mark the start codon and the stop codon of the gene. TSS->

5’CATAAA| GCAAACAGGAGATGGGCCCCAAAAGCCATGAATGGGAGCGCGTATAATCATGATTTATAATCTAGG TATCTCACTGA3’

3’GTATTT| CTTTTGTCCTCTACCCGGGGTTTTCGGTACTTACCCTCGCGCATATTAGTACTAAATATTAGATCC ATAGAGTGACT5’

Terminator sequence

Promoter sequence

TSS-> transcription start site and direction of transcription

a) Which strand of the DNA shown above, the top or the bottom, is the template strand?

b) What is the sequence of the mRNA produced from this gene? Label the 5’ and 3’ ends.

c) What is the sequence of the protein produced from the mRNA in (b)? Label the N and C termini.

d) Generate two examples each that result in a silent mutation, a missense mutation, a nonsense mutation, and a frameshift mutation for the DNA sequence above. Show the respective amino acids below each codon. (Courier font is a font typically used for sequences.)