Biology 2290F/G Lecture Notes - Lecture 10: Dynactin, Atp Hydrolysis, Axoneme
20 Apr 2017
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Microtubules are tracks for transport: come from the centrosome towards the cortex, + end always facing outside. If we look inside the cell, we see dots on the microtubules: dots move in two directions, dots represent little organelles (vesicles etc. ) Things are moved along microtubules via motor proteins. We squished out all the innards of a squid axon to experiment. Found that we need atp for motor proteins to work. Labelled proteins travel at different speeds in cells (not diffusion) We can also inject radioactive amino acids into squid axons. Take a large squid axon, inject amino acids that will be incorporated into proteins. Produces radioactive proteins in the squid axon, which will be made into all kinds of different things. Some structures made with radioactive proteins will be transported into the axon (anterograde transport) Things in section 4 are the things that have travelled the furthest from you injection site.
