Biology 2382B Study Guide - Final Guide: Talin Protein, Neurofilament, Chromatin
22 Apr 2017
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Document Summary
Technique used to grow cells under strictly controlled conditions fundamental technique for understanding biology. Sticky proteins stick cells together when you take it out of an organism, must mechanically or metabolically break it up: breaking down cell-cell and cell-matrix interactions (mechanical fragmentation, trypsine, Edta: growth factors help them grow (amino acids, vitamins, salts etc) Most cell lines come from cancers: morphology of normal vs. transformed fibroblasts (3t3 mouse fibroblasts transformed with rous sarcoma virus, normal: Elongated, aligned, orderly packed, grow in parallel arrays, contact inhibition: transformed: Rounded, hairlike processes, disorganized, grow on atop the other, loss of contact inhibition. Symmetric and asymmetric cell division: occurs during development and in adult cells. Symmetric division: mother cell will divide and create 2 identical daughter cells (same type of cell) simple eukaryotes (yeast, asymmetric, mother cell divides and creates 2 phenotypically different daughter cells. Cell type foes not contribute to function of tissue. Gives rise to cells that have very specific functions.
