Biochemistry 2280A Lecture Notes - Lecture 2: Sodium Chloride, Ammonia, Acid Dissociation Constant

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** only these 20 are used to make proteins but others can be found due to modi cations after the protein is made ** Be able to calculate the charge of amino acid groups. Be able to calculate ph and pka values. To predict the properties of a protein in solution we need to calculate the protein"s net charge. We"ll start by calculating the net charge of a single amino acid in solution. Three parts of an amino acid can carry a charge in solution: amino group, carboxylic acid group, side-chain (some amino acids) Calculating net charge of an amino acid. Need to know two things: the strength of the group to attract a proton (pka, the number of protons available in solution (ph) Ph is the symbol for the logarithm of the reciprocal of the hydrogen ion concentration in gram atoms per litre.

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