BIOL 118 Chapter Notes - Chapter 17: Poly(A)-Binding Protein, Protein Folding, Ribonuclease

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Rna polymerase synthesize an mrna version of the instructions is stored in dna. Only one strand of dna is the template. Other strand is the non-template or coding strand. Matches the sequence of the mrna (except u or t) Fig 17. 1: rna transcription - catalyzed by rna polymerase - formation of phosphodiester linkages between base pairs of the rna. Rna polymerase perform template directed synthesis in the 5" to 3" direction. Rna polymerase do not require a primer to begin transcription. Eukaryotes have at least 3 distinct types. Rna polymerase cannot initiate transcription on its own. In bacteria, sigma protein must bind to it first * doesnt need primer but uses sigma protein as a coenzyme. Rna polymerase and sigma form a holoenzyme. Rna polymerase is the core enzyme because it has the active site. Sigma binds to dna segments called promoters that promote the start of transcription. Different sigma proteins bind to promoters with different dna base sequences.

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