BISC403 Lecture Notes - Lecture 17: Brca2, Cytoskeleton, Dna Mismatch Repair
28 Apr 2017
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Document Summary
Proto-oncogenes are normal; there is a mutation and you get an oncogene. Transition from g1 to s is the most efficient place to stop before it begins replicating and going through the process. Even after replication, there can be errors in the dna; genes that are supposed to fix these errors: dna pol has proofreading mechanism. Identifying oncogenes through cell transformation assays: human gene that is oncogenic can be identified and cloned from transformed mouse cells, if the gene transforms the mouse cells, it"s conformation that it"s an oncogene. Hpv pathophysiology: hpv effects, oncoproteins, e6 binds p53 blocking apoptosis, e7 releases e2f from prb driving cells into cycle. The ras oncogene is the mutant form of the ras proto-oncogene: normal ras is inactive until it becomes activated by binding of growth factors to their receptors, oncogenic forms of ras are constitutively activated.
