BIOL 367 Chapter Notes - Chapter 15: Wild Type, Cleavage And Polyadenylation Specificity Factor, Intron

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1 May 2017
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Chapter 15 rna processing ii: capping and polyadenylation. Cap1 is the same as the cap shown in this figure. Cap (cid:1006) has a(cid:374)othe(cid:396) (cid:1006)"-o-methylated nucleotide (two in a row) Cap2 is found only in eukaryotic cells, cap 1 is found in both cellular and viral rnas and cap0 is found only in certain viral rnas. Synthesis: a nucleotide phosphohydrolase (also called rna triphosphatase) clips the gamma-phosphate off the t(cid:396)iphosphate at the 5" e(cid:374)d of the growing. The cap is joined to the rest of the mrna through a triphosphate linkage found nowhere else in the rna. Thecap might therefore be expected to protect the mrna from attack by rnases that begin at the 5" e(cid:374)d of thei(cid:396) su(cid:271)st(cid:396)ates a(cid:374)d that (cid:272)a(cid:374)(cid:374)ot (cid:272)leave t(cid:396)iphosphate li(cid:374)kages. Eukarotic mrna gains access to the ribosome for translation via a cap-binding protein that recognizes the cap. If there is no cap, the cap-binding protein cannot bind and the mrna is very poorly translated.

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