MCDB 423 Lecture Notes - Lecture 38: Tubulin, Beta Cell, Long-Term Memory

• School: U of M
• Department: Molecular, Cellular and Developmental Biology
• Course: MCDB 423
• Professor: John Kuwada

Document Summary

So fru expression is important for mating behaviors in flies. There are subsets of neurons that express fru. This suggests that it (cid:373)ust (cid:271)e the a(cid:272)ti(cid:448)it(cid:455) of the fru (cid:374)euro(cid:374)s that"s (cid:374)e(cid:272)essar(cid:455) for (cid:272)ourtship behaviors in males. Utilized a mutation called shibire which is a geen required for synaptic vesicle recycling. So in a shi mutant fly, the recy(cid:272)li(cid:374)g does(cid:374)"t o(cid:272)(cid:272)ur. So eventually the terminals will run out of synaptic vesicles and now the synaptic transmission is eliminated. There is a temperature sensitive shi mutation that is dominant. If you drive gal4 in fru+ neurons and you are uas-shi temperature sensitive, you can block synatpic transmission with temperature. At high temperature, the shi function is blocked. So you can induce inhibition of synaptic release by using genetics and temperature. I(cid:374) a tra(cid:374)sge(cid:374)eti(cid:272) fl(cid:455) that"s fru-gal4 and uas-shi mutant, at high temperature the courtship index decreases.