MCDB 423 Lecture Notes - Lecture 31: Laminin, Agrin, Basal Lamina

• School: U of M
• Department: Molecular, Cellular and Developmental Biology
• Course: MCDB 423
• Professor: John Kuwada

Document Summary

Alpha-bgt (cid:271)lo(cid:272)ks all nmj fu(cid:374)(cid:272)tio(cid:374) so you"ll stop (cid:271)(cid:396)eathi(cid:374)g at (cid:448)e(cid:396)y lo(cid:449) (cid:272)o(cid:374)(cid:272)e(cid:374)t(cid:396)atio(cid:374)s of the toxin and die (irreversible, highly specific reaction to achr and is used as a dye to label the. You see that the clusters of the achr are right under the pre-synaptic neuron which makes sense. In a mouse which the motor neurons have never developed due to a mutation called hb9 (critical transcription factor needed for the differentiation of motor neurons) So thei(cid:396) de(cid:448)elop(cid:373)e(cid:374)t does(cid:374)"t (cid:396)e(cid:395)ui(cid:396)e the p(cid:396)e-synaptic patter. However the pattern of the achr is no longer restricted to a tight little zone as in the wt. So a signal from the pre-synaptic neuron regulates some aspect of muscle development. Fibers form by a number of myoblast (which are mono-nucleated) will fuse together to form a larger muscle cell. In the synaptic folds, you have the addition of ache which are made and secreted by the muscle fiber.