BISC300 Chapter Notes - Chapter 17: Merck & Co., Polyacrylamide Gel Electrophoresis, Streptavidin
8 May 2017
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17. 1: key discoveries led to the development of recombinant dna technology. Recombinant dna is dna with a new nucleotide sequence. Such dna is formed by joining fragments from two or more different sources. These enzymes, known as restriction enzymes or restriction endonucleases, recognize and cleave specific sequences about four to eight base pairs long (figure 17. 2). Restriction enzymes identify specific dna sequences called recognition sites. Each restriction enzyme has its own recognition site. Hundreds of different restriction enzymes have been purified and are commercially available. When ecori cleaves between the g and a residues, unpaired 5 -aattc-3 remains at the end of each strand. They isolated the enzyme reverse transcriptase (rt) from retroviruses. Rna genome that is copied into dna prior to replication. The mechanism by which reverse transcriptase accomplishes this is outlined in figure 17. 5. Processed mrna can be used as a template for complementary dna (cdna) synthesis in vitro.
