MIC 201 Study Guide - Midterm Guide: Microscope Slide, Asepsis, Gram Staining
10 May 2017
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Objectives: be aware of the abundance of microorganisms in our environment, practice prep of a smear on microscope slide, simple staining, practice care and use of microscope, view cell morphology of various microorganisms. In order to survive there must be proper growth conditions: Could not divide under normal growth condition. Stains are used to enable microscopic visualization of shape, appearance, and arrangements of cells. Preparation of smears - a suspension of cells in water which is dried on a slide. Should be light and uniform in appearance for best results. Simple staining - bacterial smears with a single dye: heat loop, water on slide by loop, heat loop again, get bacteria and mix it with water, let it air dry, fix the smear with heat 2-3x. Stain: few drops of stain, give one minute to react, rinse with water immediately, blot with paper towels, careful not to over stain.
