Biology 1202B Lecture Notes - Lecture 16: Dna Ligase, Sticky And Blunt Ends, Restriction Enzyme

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Biotechnology technology applied to biological systems or living organisms to make or modify products or processes for a specific purpose. Re recognize short amount of dna sequence (4-8nt long) called restriction sites and cut the dna at specific locations within those sequences with a restriction enzyme to produce restriction fragments. Restriction sites are often palindromes same sequence 5" to 3" from both strands and produces sticky ends: ex. Sticky end can form hydrogen bonds with complimentary sticky ends on any other dna molecules cut with the same enzyme. Complete digestion conditions are such that if a restriction enzyme site is present in the dsdna, the enzyme will cut at all the dsdna sites. Incomplete digestion conditions are such that if a restriction enzyme site is present in the dsdna, the enzyme may cut at all the dsdna sites: conditions include things like temperature, amount of time etc Each human cell has two copies of most genes.

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