BIO130H1 Study Guide - Final Guide: Electrochemical Gradient, Golgi Apparatus, Membrane Protein
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BIO130H1 Full Course Notes
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Document Summary
In cell, outside of nucleus , fluids and organelles. No repeating unit patter or patch, no specific arrangement, everything moves separately: lipid bilayer. Certain intracellular signaling proteins need to bind to specific inner leaflet pplpds. Artificial bilayers: liposomes: study permeability, study membrane proteins, delivery into cells. Tails:14-14 carbons, saturated or unsaturated(kinks, cis double bond) Diffuses laterally, rarely flipflops (only with help of pplpd translacator) Decreased mobility of pplpd tails: less permeable membrane. Eg. receptors influenced by outside channels for transport. Saturation of pplpds : cis bonds help presernve fluidity. Length of pplpd tails: shorter, more fuild b/c less interactions. Double membranes: nucleus and mitochondrion, both have intermembrane space. Lumens of membrane bound organelle faces exoplasmic face. Eg, glycolipids found on outer leaflet of plasma membrane but synthesized on lumen face of golgi. Addition of phospholipdis to isolated proteins, removal of detergents and creation of minicells.
