BIOL303 Study Guide - Lateral Plate Mesoderm, Apical Ectodermal Ridge, Fgf10
18 May 2013
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Aaron: define proximal-distal, anterior-posterior and dorsal-ventral limb axis. Paracrine factor that act as inductive signal for limb formation. Assist in fgf loop by inducing formation of fgf8. Positive loop with fgf8 (stimulate production of fgf8) Aaron: describe the roles of fgfs, tbx5 and tbx4 during limb bud formation in the chick. Outline the experiments which demonstrated the roles of the above proteins. Induce formation of aer and help maintain mitosis of cells within it. Use of fgf bead that was put into area b/w tbx genes induced formation of chimeric limb. Half of limb became hind, half became fore depending on location (affected by tbx) Use of in situ hybridization label complementary rna strand that binds to target rna (tbx) which allows coloured location of protein expression. Aaron: outline the experiments which showed that the specificity of the proximal-distal limb axis is determined by the limb mesoderm and not by the aer.
