BIO120H1 Lecture Notes - Lecture 7: Multiple Sequence Alignment, Bioinformatics, Clustal
36
BIO120H1 Full Course Notes
Verified Note
36 documents
Document Summary
Lecture 7: what is gene & where do i find it? (part ii) For genome projects, gene prediction is often difficult. Which of the following factors is/are not a concern: alternative splicing, the degeneracy of the genetic code, possible presence of introns, variability in consensus sequences for transcription initiation factors, none of the above. Alternative splicing complicates gene prediction b/c final spliced product can vary in different tissues. Degenerate refers to the fact that dna sequences don"t code to greatest capacity as in 64 codons but we only have 20 aas and some function as stop codons. Possible presence of introns can disrupt the reading frame resulting in frame shifts which may cause stop codons that may actually occur prematurely, introns is huge problem for predicting genes. Phylogenetic analysis (looking for relatedness in a family tree) Bioinformatics on one hand can tell certain amount of things, but complementary to them are the experimental investigations in the laboratory.
