BIO120H1 Lecture Notes - Lecture 5: Okazaki Fragments, Dideoxynucleotide, Dna Sequencing

42 views5 pages
28 May 2013
School
Department
Course
shdhhfhshhpla3806 and 40102 others unlocked
BIO120H1 Full Course Notes
36
BIO120H1 Full Course Notes
Verified Note
36 documents

Document Summary

Lecture outline: dideoxy chain termination method, manual, automated, genome databases, blotting. Which of statements regarding dna replication is correct: no enzyme that catalyzes 3" to 5" nucleotide polymerization has ever been found. Steps: anneal primer, extension by dna polymerase (to chains of varying lengths, run gel (separate out those chains of varying lengths) In an in vitro setting (lab), can use dna polymerase to extend, from template, nascent chains of varying lengths. Types of nucleotide: dntp ddntp exactly same except they lack 3" -oh group (does not allow nucleic acid chain to elongate any further b/c polymerase requires that for further extension for formation of phosphodiester bond) Blue is primer designed primer so it is complementary to template. In test tube, have template dna, primers, dntps & ddntps. Do reaction in 4 test tubes, run it out on gel gel separates fragments based on size smaller fragments move faster.