MGY299Y1 Lecture Notes - Yeast Artificial Chromosome, Human Genome Project, Karyotype
3 Jun 2013
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The human genome project traits could in theory be mapped in genome; feasible to track genes inherited in mendelian fashion; how useful complete maps of genome would be; difficult task to sequence; Take rna convert to cdna, sequence allow functional analysis, which was the major interest. Select model organisms some already partially mapped for example, c elegans. Genome project purpose to have multi-purpose maps understand genome. Local chromosome regions sub region of chromosome to get good detail of the map merge chromosome maps together to get full picture. Top four methods broad resolution poor resolution but looking at whole or portions of chromosomes. Tools that came about whole chromosomes giemsa banding . Somatic cell hybrids maintains a human chromosome in the dna of the cell line (usually mouse, hamster) chromosome may rearrange. Start from top of shorter arm, position arm go down chromosome. One interesting fact is that chromosome 1 is not bigger than 21.
