MGY440H1 Lecture Notes - Genistein, Reoviridae, Dimethyl Sulfoxide
3 Jun 2013
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Genistein inhibits multiple tyrosine kinases, perhaps the reason. Src kinase mediates productive endocytic sorting of reovirus. Figure 4 - bind to jama and integrins, endocytosis, proteolytically cleaved, outer capsid off, mu 1 gets cleaved twice, endosome breaks, release. Genistein inhibits all tyrpsine kinases; pp2 inhibits src (tyrosine kinase) Fluorescent focus assay - stain cells for reovirus by antibody - if cells lit up, infected cell. A and b shows that src required for infectivity. C - measure viral titre - 10x decrease comparig dmso and genistein and. D - to prove not cytotoxicity of compounds - tryphan blue assay - pp2 number of cells comparable to dmso; genistein decreased number of cells due to cell proliferation injured for decreased. A - twenty hours = one replication cycle - after one hour of adsorption, the virus infectivity unchanged from untreated cells. B - isvps show no increase in infectivity - note that virion entry and isvp entry are not the same.
