MGY440H1 Lecture Notes - Lytic Cycle, Poliovirus, Interferon
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3 Jun 2013
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Showed that wild-type ires active in multiple tissues. Sabin ires also active in multiple tissues but to lesser degree in every single tissue. Sabin ires didnt work as well - active in cns. Using newborn mice, sabin strains able to replicate and kill. Different approach than kauder and raccaniello several host factors that bind - looking at polypyridmine binding tract protein. Sabin scenario in cns - translation conpromised, lower yield, less spread - means immune system can clear the infection before the virus has killed too many neurons - key: before virus has killed off too many. In embryos by electroporating - measured amount of virus they got from wild- type from sabin strain and ten hours afterewards, 100 times more virus for wildtype than sabin strain - so difference in replication. With sabin ires - have compromised binding of ptb and have low. This means inefficient translation of the sabin ires and.
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