MGY440H1 Lecture Notes - Interferon Gamma, Interferon Type I, Influenza A Virus Subtype H5N1
3 Jun 2013
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Paper: influenza h5n1 and h1n1 virus replication and innate immune responses . Cells in differentiated and undifferentiated states may account for conflicting results in previous papers. Nhbe cells grown on transwell inserts: cells with medium below, and air above, to infect, virus on top. Looking at differentiation of cells: day 21 see cilia and pseudocolumnar cells unlike at day 7, well-differentiated (21 days) can be used as good model to model human bronchial tissue. Why is h5n1 so virulent: even in the same cells, using cells that can differentiate, state of differentiation makes a difference. First two rows are undifferentiated epithelium: mal1 unpreferred h5n1 sialic receptor 2-3 gal b1-4 glcnac, mal2 preferred h5n1 sialic 1-3 gal, sna sia 2-6. Receptors required for infection of differentiated and undifferentiated tissues are present but in varying quantities but present in both types. Influenza infection in both differentiated and undifferentiated cells. Target cells to be infected staining of viruses.
