MGY440H1 Lecture Notes - Journal Of Virology, Rna Virus, Reverse Genetics
3 Jun 2013
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Document Summary
For the other influenza viruses, ns1 associated with virulence. Reverse genetics = looking at the effect of a mutation you generate. In the context of influenza virus a negative strand rna virus cell cannot process negative- sense rna. Two main systems - 12 plasmid system and 8 plasmid system. Initially 17 plasmid system did not need the structural proteins (in square brackets) just needed polymerase proteins. Consist of set of plasmids that express the virion rna all have rna polymerase i promoter to give virion rna in cell. Cell transfected with mixture of these plasmids (8 + 4: 8 virion rnas and get polymerase proteins which copy the virion rna into mrna = giving rise to 10 mrna (two are spliced) Virion rna copied by appropriate polymerase to give mrna translated into more proteins. Not all segments are equally compatible with each other . Terminology: pathogenicity ability to cause disease, pathogenesis mechanism for disease development, virulence severity of disease.
