Biology 2581B Lecture Notes - Lecture 10: Fibrinogen, Plasmid, Shotgun Sequencing

49 views3 pages

Document Summary

In order to clone a gene without mapping information, we need information about family history and an idea of what might be causing the mutation and what its protein might be (biological as well as genetic information). Hemophilia a cloning & the blood clotting cascade: In this positional cloning example, we wish to find the gene causing hemophilia a, where patients of this disease fail to clot their blood (wounds keep on bleeding). In this same blood clotting cascade, coagulation factor ii (aka prothrombin) is cleaved to become thrombin (a serine protease), which then converts fibrinogen (soluble glycoprotein that doesn"t clot) to fibrin (insoluble fibre which does clot through cross-linkage). Blood tests can determine whether an active form of each factor involved in the clotting cascade is present. The results of such analyses show that many hemophiliacs, lack an active factor viii in their blood. Colony hybridization & the positional cloning of hemophilia a:

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents