MIC2011 Study Guide - Final Guide: Macconkey Agar, Gram Staining, Bile Acid
Document Summary
O staphylococcus aureus could be present/colonise the human body. Persistent carriers; carry one type of strain of the population (20%) Intermittent carriers; form the large part of the population (60%) Name the organism that is identified using the bactistaph. Staphylococcus genus: a single colony consists of identical cells and can be used to provide subsequent pure cultures of that species in broth/agar media, the inoculated area forms the primary inoculum. Explain why agar is used in preference to gelatin. Agar is easier to extract and it has a higher melting point. Explain the purpose of passing the dried smear through the bunsen flame. Kill the cell, and make it stick to the slide. Alters the cells so it can readily accept the stains/dyes. Suggest the effect on the gram stain if a whole colony is used to make the smear. If you use too many cells, you won"t be able to see as well, or even at all.
