BIO 190 Chapter Notes - Chapter 5: Rolling Circle Replication, Hfr Cell, Centimorgan

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16 Sep 2013
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They are fast-dividing, take up little space, convenient. They can be culture on liquid or solid surface as long as basic nutrients are provided. Plating is done with a sterile spreader and once the cell mass is more than 107 it forms a colony. Unlike wild types, thse can grow even in the presence of an inhibitor. Two cells that were auxotrophic for different nutrients were mixed and the mixture was plated and compared with controls of only a and b cells. Some of the mixture cells grew into colonies to produce prototrophs showing that some form of recombination of genes must have ocured between the two different types of cells. It waqs determined that physical contact was needed for wt to form and this was determined to be conjugation uunder an electron microscope. It was determined later that one cell acts as the recipient and the other as a donor.