BIO 190 Chapter Notes - Chapter 1: Reverse Genetics, Enzyme, Nucleolus Organizer Region
16 Sep 2013
School
Department
Course
Professor
Document Summary
Point of interest =>mutants affect in process e. g. irradiation => find the gene => fetermine the function by studying gene and encoding region. Start with gene of interest (comparing dna sequence from known protein to unknown) => mutate that gene => determine the function. Dna fragment is taken adn repoialted many times over untuk there are many copies. Fragments of the genome are obtained by cutting the dna by vigorous agitation, scissoring with certain enzymes, etc. The vectors with their loads are introduced to bacterial cells. The vector replicated as the cell divides until it becomes a colony containing clones. Representative system of subject in question e. g. yeast for gustone study, bacteria for dna. Dna from the organism in study is extracte dand cut with enzymes at specific target sequences. Enzyme cuts genome into defined populations of segments of specific sizes. The gragments are separated into groups of fragments of different sizes (fractionated) using electropheresis.
