BIOL 205 Lecture Notes - Lecture 16: World Federation Of Trade Unions, Hydrolysis, Prokaryotic Large Ribosomal Subunit
27 Sep 2013
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Nov 28/2011 lecture 16: visualizing gene expression. Genetic screens: wieschaus, e. lewis, c. nusslein- Used to visualize mrna transcripts cdna clone of a gene is transcribed to obtain a ssrna probe. Embryos are incubated with rna probe and any unbound probe is washed away. Enzyme conjugated antibodies are added and mrna expression is visualized under light microscope. Cdna clone of a gene is expressed in bacteria to produce protein which is injected into ivertebrate host. Antibodies to protein are produced (igg) and this is incubated with the embryo. Fluorochrome conjugated antibody is added to igg of host species. The final expression of hox genes in adult depends on control of their expression early in embryogenesis. Hox proteins have a sequence in common, called ahomeodomainwhere these. 60 amino acids are found in all homeotic genes and code for dna binding proteins. This may have arisen by tandem gene duplication of an ancestral gene.
