BIO 325 Lecture Notes - Genetic Linkage, Wild Type, Point Mutation
29 Sep 2013
School
Department
Course
Professor
Document Summary
F factor is integrated in the bacteria chromosome. This type of conjugation can be used for gene mapping with the origin [interrupted mapping --- time map] F: during excision, it has brought tiny amount of bacteria chromosome. R plasmid - drug resistance plasmid; can be transferred from one bacteria to another through conjugation. Col plasmid - in the e. coli; colicin enzyme. Transformation - small pieces of extracellular dna are taken up by a living bacterial cell and integrated stably into the chromosome. 2 events that happen independently: entry of dna (may happen) If it"s a plasmid, it doesn"t need integration --- will free survive in the cytoplasm since they can auto replicate. But if it"s a small strand of dna and didn"t get integrated, then it will be gone. Recombinant region contains one host strand (present originally) and one mutant strand. Because these strands are from different sources, this region is referred to heteroduplex.
