BIOL 342 Study Guide - Midterm Guide: Ribonuclease H, Nitrocellulose, Apyrase

43 views23 pages

magentazebra977

16 Oct 2011

School

Department

Course

Professor

For unlimited access to Study Guides, a Grade+ subscription is required.

Document Summary

Biotechnology use of organisms (their metabolism) to produce a product for a commercial use. Molecular biotechnology dna manipulation to enhance a natural product (protein/enzymes) e. g. insertion of human insulin into e. coli for insulin production. Cohen, chang, and boyer s work first demonstration of gene cloning using plasmids as. However, thought to be dangerous if genes combined from two different organisms produced a novel organism with undesirable and dangerous properties vectors. Mrna modified (5 methylated cap and 3 . Ribosome binds 5 cap, scans mrna to aug then begins translation. Gene: is a specific nucleotide sequence containing the elements required to produce rna or protein. Dna 5 po4 p o rbs atg tag rbs atg tag t oh 3 mrna 5 po4 sd aug uag rbs aug uag oh 3 . Protein nh2 n-f-met (first aa) aa-aa-aa-aa-cooh (last amino acid) Operon: cluster of genes that are regulated and under the control of an operator.

Related Questions

Background:

Biotechnology allows the use of living organisms or their processesfor human needs or purposes. Currently, this topic includes suchgeneral examples as cloning, stem cells (adult, umbilical cord, andembryonic), DNA fingerprinting, biological warfare, bioremediation,genetically modified organisms, vaccines, and transgenic plants andanimals to name a few. In this assignment, you will explorespecific examples of biotechnology applications.

Assignment details:

Research how a SPECIFIC biotechnology application within the aboveGENERAL examples (or ones not listed above) is used today. Example:Insulin production in bacteria is an example under the generaltopic of recombinant organisms.

What specific biotechnology application did you choose?
Briefly describe this biotechnology application. How is itdone?
Based upon the definition of biotechnology in the assignment,describe how your biotechnology application is an example ofbiotechnology.
What benefits are derived from this application?
Are there any concerns or negative impacts associated with this useof biotechnology? Why or why not?

champagnetrout780

1.Place the following in order from largest to smallest:

1. nitrogenous base

2. chromosome

3. nucleus

4. gene

5. nucleotide

2.Plasmids used for recombinant DNA work typically contain a gene for resistance to some type of antibiotic. The reason for this:

a.Helps you select out bacterial cells that have taken up the plasmid by

incubating them in the presence of the antibiotic

b.Helps make sure your culture doesnât get contaminated with viruses or fungi

c.Helps protect biotechnology lab workers from becoming infected with the bacteria

d.Helps make new types of antibiotic-resistant bacteria that will causewidespread disease and give the biotechnology industry new drugs to manufacture

3.Put these events in the correct sequence:

1-translation

2-mRNA splicing

3-transcription

4.A human protein product requires modifications in the endoplasmic reticulum and Golgi. Which of the following is true?

a.Bacteria will be able to produce this product, since they contain an endoplasmic reticulum and Golgi.

b.Bacteria will not be able to produce this product properly, because they lack an endoplasmic reticulum and Golgi.

c.This product should be produced in a virus, which is the least expensiveeukaryote that can be grown.

d.No cell type can produce this protein, so the company needs to move on to a different product.

1.Describe and provide an example of gene regulation in eukaryotic organisms at the level of

a. Chromosome

b. Gene transcription

c. Posttranscriptional processing

d.Translation

e.Posttranslational processing

2. Explain the role that gene regulation plays in the formation of a multicellular organism with cells of different functions from a single cell.

whitecricket273

BIOL 342 Study Guide - Midterm Guide: Ribonuclease H, Nitrocellulose, Apyrase

Document Summary

Get access

Related Documents

BIOL342 Lecture Notes - Corepressor, Antihistamine, Proline

BIOL342 Study Guide - Reca, Cosmid, Pyrosequencing

Related Questions