BIO 2133 Midterm: Midterm #1 Review Modules 1 and 2 notes

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crimsonoyster634

16 Oct 2011

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Document Summary

Forward genetics: forward genetics is an approach that encompasses several means of identifying the gene or set of genes that are responsible for a particular phenotype within an organism. Mutant isolation is followed be defining genes pathways, cloning the gene, and creating more mutants in order to understand the biological pathways. (mutants define the normal gene. ) Reverse genetics: reverse genetics is an approach to discovering the function of a gene by analyzing the phenotypic effects of specific gene sequences obtained by dna sequencing. This approach starts with a dna sequence (probably learned from a genome sequence, a single gene obtained from a gene bank, or by dna sequencing) that has no known function and then attempts to find that function. Simply put, while forward genetics seeks to find the genetic basis of a phenotype or trait, reverse genetics seeks to find what phenotypes arise as a result of particular genes.

Related Questions

A UQ PhD student discovered a mutant in maize called roundcob1 (rc1). She was only able to find one recessive allele of rc1, which she called rc1-1. Your honours project is to identify new recessive mutant alleles of rc1 (e.g rc1-2, rc1-3...) using a mutant screen.

a) Describe a forward genetics approach youâd take to identify the new rc1 alleles using EMS mutagenesis, and by screening the cob phenotype of the M1 plants. Make sure you specify the genotypes (genetic background) of the parental line(s) you will use in your screen and briefly outline your screen design

b) Specify how you would use a genetic test to prove that your M1 plants carry new recessive mutations in rc1.

yellowlizard809

In order to determine the probable function of a particularsequence of DNA in humans, what would NOTbe the most reasonable approach?
Perform a Blast search to see if this sequence is similar to genesin other organisms
Create an anti-sense RNA and inject it in cells into a person tosee its effect on phenotype
Genetically engineer a mouse with a copy of this sequence andexamine its phenotype.
Prepare a knockout mouse without a copy of this sequence "knockedout" and examine the mouse phenotype for theconsequences.