CSB349H1 Lecture Notes - Lecture 6: Dna-Binding Domain, Tata-Binding Protein, Deoxyribonuclease I

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coraleel39

16 Nov 2013

School

UTSG

Department

Cell and Systems Biology

Course

CSB349H1

Professor

Alan Moses

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Document Summary

Csb349 lecture 6 transcription ii: promoters and sequence-specific dna binding proteins. Slide 2 regulating gene expression: the role of promoters. The promoter is upstream of the transcription start. There are two major parts of the promoter: core promoter. Contains dna recognition elements for the basal transcriptional machinery that is going to be involved in transcription start: proximal promoter. A collection of regulatory elements that are important for gene regulation. All kinds of different elements can appear in the proximal promoter region. This is where regulatory sequences are found. Slide 3 experimental characterization of functional promoters. There is no well understood language or code of what makes the promoter work. We don"t know what the important elements are in the promoter. The classical experiment for studying promoters is called promoter bashing: break the promoter into pieces, figure out which ones work and which ones don"t, which will tell us where the functional sequences are.

Related Questions

How are enhancers different from promoters in affecting gene transcription in eukaryotes? Make sure that you include in your discussion: (a) their relative position to the transcription initiation site; (b) the role of binding sites for proteins; (c) how each affects initiation and transcriptional regulation (in the latter, make a distinction between the core and the regulatory promoter)

sangriafly705

In Eukaryotic transcription, what is the difference between an enhancer, a mediator, a coactivator and a trascriptional activator protein? In your answer, for each one include (a) if it's the term refers to a DNA sequence or a protein, and if it's a protein (b) if it interacts with DNA, with another protein, or with both. Also how are enhancers different from promoters in affecting gene transcription in eukaryotes? Make sure that you include in your discussion: (c) their relative position to the transcription initiation site; (d) the role of binding sites for proteins; (e) how each affects initiation and transcriptional regulation (in the latter, make a distinction between the core and the regulatory promoter

lilacseal677

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

CSB349H1 Lecture Notes - Lecture 6: Dna-Binding Domain, Tata-Binding Protein, Deoxyribonuclease I

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