LIFESCI 2A03 Lecture Notes - Lecture 9: Hematopoietic Stem Cell, Cd90, Transdifferentiation
19 Nov 2013
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Not full restoration of pluripotency; go to less differentiated stage. Eg/ blood cell haemopoetic sc different blood cell: transdifferentiation switch to another cell type lineage. Examples of transdifferentiation (1998-2004) reports were made of various differentiated tissues containing cells derived from bone marrow grafts: radiation to destroy bone marrow (mice); ultimately lethal if animals cannot replace blood cells and immune cells. Labeled hsc become not only blood cells, but other cells as well. Donor cells had fused with host cells (genetic marker of the donor was still displayed in the fused cells) Importance of these studies transdifferenetiation is much easier than going back to pluripotent state to differentiate into different cell types. Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Report a combination of three developmental transcription factors (i. e. gata4, mef2c, tbx5) rapidly and efficiently reprogrammed postnatal cardiac or dermal fibroblasts directly into differentiated cardiomyocyte-like cells. Fibroblast to cardiac myocyte: cardiac fibroblast provide support structure and secrete signals.
