BIOC 301 Study Guide - Thermal Cycler, Palindromic Sequence, Restriction Enzyme
Document Summary
The overshare-hydrolase(ovr-h) enzyme is inactivated in people who luv fb & twitter. You postulate that this inactive (ina) form is different from the normal, wild-type (wt) form at the gene level and thus design an experiment to check this. Using the gene information below write out the primers you will use in your pcr reactions, 8mers should be sufficient here. wt ovr-h. Knowing that the restriction endonuclease noti cleaves after the first gc of the palindromic sequence 5"-gcggccgc, describe an experiment which would allow you to differentiate between inactive and active forms starting from. Your instructor has assigned you to clean up after a lousy ta who did not save pcr programs. You are to program the thermocycler so the bioc420 class can proceed with their experiments this afternoon. In his notebook, you find some chicken-scratch and decipher the following: You suspect this information is out of order and re-input correctly into the thermocycler:
