BIOL240 Study Guide - Final Guide: Gram Staining, Staphylococcus Epidermidis, Petri Dish
16 Dec 2013
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Lab safety: inoculating loop/needle should be heated to a red-hot stage. Working with microorganisms classified as biosafety level 2- class 2 microorganisms in lab manual found with bold and asterisk; a class 2 safety cabinet used in lab (has hepa filters) Use of bacti-cinerator: inoculating loop and needle sterilizer- used when working with level 2 pathogen. They"re designed to eliminate aerosols (potential source of contamination). Wait 10 mins after incinerator is on for optimal sterilizing temperature. Keep loop in heater for minimum of 10 seconds; microorganisms should be destroyed in. 10-15 seconds; don"t need a glowing loop to ensure sterility. Fire and explosion: fire extinguishers: types of fires: a-ordinary combustibles, b- flammable liquids, c- electrical fires, Never use water on electrical fires (type c: carbon dioxide extinguishers- used on most fires (types a,b,c) except on sodium, potassium, magnesium and aluminum (type d, dry chemical extinguishers- good for flammable liquids and electrical fires (types a,b,c)
