BIO372H5 Lecture Notes - Electrophoresis, Logarithmic Scale, Reptation
Document Summary
Studying the scheme, why things are the way they are. 1859 - charles darwin: a lot of molecular patterns are strongly influenced by natural selection. 1869 - dna - no idea of the function yet. 1931 - there is crossing over between chromosomes, and recombination occurs. 1944 - a huge discovery, biochemistry is linked to the genes. 1958 - semi conservative dna - another classic experiment. 1996 - many investigators - eukaryotic genome - very expensive, but now is cheaper. Dna is applied to gel, apply a current (there is a voltage) positive terminal is down, dna is negatively charged, the speed is relative to the size, we can only measure down to 50 (anything smaller we can"t measure) There is variation because of error in measurement. Electrophoretic mobility of dna fragments of various sizes. The electrophoretic mobility (migration rate) of a dna fragment is inversely proportional to the log of its size.
