CSB328H1 Lecture Notes - Lecture 3: Ornithine, Disaccharide, Autolysin
25 Jan 2014
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Mgy377h lisa zhao 2013 | page 1. L e c t u r e 3 : b a c t e r i a l c e l l w a l l. 1. in gm+ bacteria, as much as 90% of the cell wall consists of peptidoglycan layers (up to ~30 layers) in gm- bacteria, only ~10% of cell wall is peptidoglycan (1 layer) Peptidoglycan (murein: chemically unique, rigid structural component of cell wall found in bacteria. 1. low solute condition w/o pg, imbalance allows h2o to enter cell. 1. lysozyme is added in buffer for lab experiments requiring extraction of bacteria proteins. 2. isotonic solution lysozyme digests pg, but om remains intact: contains compounds unique to the microbial world: -amino acids, diaminopilemic acid (dap), n-acetyl muramic acid. Mgy377h lisa zhao 2013 | page 2. Alanine: unusual -amino acids (config is determined by amino acid location. Mgy377h lisa zhao 2013 | page 4.
