CSB328H1 Study Guide - Cyclopia, Sertoli Cell, Laminin

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Reading: gilbert chapter 3, page 136 (in chapter 4) Csb328h1 lisa zhao 2013 | page 1 (ectodermal) Just cell types have diff cell membrane components these diff components enables formation of organs. Townes & holtfreter (1955) discovered segregation of cell types after dissociation. Prepared single-celled suspensions of each germ layer & combined them. Cells were separated in alkaline solns for separation. When cells of diff germ layers were combined, & ph normalized, cells stuck together. Selective affinity: inner surface of ectoderm has +ve affinity for mesodermal cells. Ve affinity for endodermal cells: mesoderm has +ve affinity for ectodermal and endodermal cells, selective affinities change during dvpmt. Cells interact diff w each other at diff times. Segregation of tissue cells (w trypsin) from later-stage animals reaggregated to form tissue-like. Endoderm arrangements (moscona 1961; giudice & just 1962) Page 2 | csb328h1 lisa zhao 2013.

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