BIOL273L Study Guide - Final Guide: Cerebral Hypoxia, Valsalva Maneuver, Surface Tension
31 Jan 2014
School
Department
Course
Professor
Document Summary
Generation of electrical potentials by an artifical membrane. Rmp arises because the cell membrane is more permeable to some ions than others. Na+ more outside (restricted) can"t pass as easily. = positive charge on outside of cell (rmp). Ap/gp disturb charge difference (reduce, increase, reverse) send sensory info to s-cord, brain to send signals to muscles and organs. Ussing chamber membrane only permeable to na/k (cations) not anions (cl/i). Cations diffuse down their concentration gradient = difference in charge. Poured some solution in, rinsed the chamber with it. 60ml then of each solution into each chamber. Important to fill them at the same time so minimum pressure is applied to the membrane. Measuring chamber 2 with respect to chamber 1, voltage should read negative (black in chamber 1); these electrodes will always go into the same chambers. Aeration ensure good mixing of solutions, prevent unstirred layers of solution next to membrane.
