BIOL 1010 Lecture Notes - Fibronectin, Action Potential, Cytokinesis
Document Summary
The invention of the light microscope led to the discovery of cells. Three important concepts in microscopy are magnification, resolution and contrast. Magnification = ratio of size of object image to real size of object: how much larger an object is made to appear compared to its real size. Contrast: accentuate different parts of the specimen. Types of microscopes: light microscope, visible light is focused on the specimen with a condenser, light passing through the specimen is refracted through an objective lens and an ocular lens producing an inverted magnified image. 1000 x magnification and 0. 2 m resolution conventional light microscopy such as brightfield microscopy. (limited by wavelength of visible light) for: above 1000 x magnification objects become increasingly blurry this is often referred to as. Page (cid:0: electron microscopes have a much higher resolving power. Theoretically it is around 2 pm for transmission electron microscopy due to the much shorter wavelength of the electron beam.
