MCB 3020L Lecture Notes - Phosphate, Osmoregulation, Resazurin

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Diagnostic mirobiology culture and isolation of microbes. An individual species has it"s own distinct fingerprint ; it will produce certain results to different tests, usually resulting in a combination of outcomes unique to that individual species. These difference arise from the fact each microbe has adapted to it"s own micro-environment, and since each micro-environment, for all intents and purposes, is unique, it has developed certain sensitives and resistances to each variable found. The purpose of this lab is to utilize the various biochemical techniques and selective/differential media in order to analyze and determine an unknown species. The reactivity of our species to each test will be recorded which will help us learn how to use the gideon database to identify a species. Because the species were originally cultured in the classroom, one can assume species that thrive in low ph, extremely low and extremely high temperatures, and that are anaerobic will not be found.

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