BLG 10A/B Chapter Notes - Chapter 4: Chondroitin Sulfate, Serous Membrane, Mast Cell
1 Mar 2014
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Document Summary
Preparing human tissue for microscopy: specimen must be fixed (preserved, cut into sections (slices) thin enough to transmit light/electrons, stained to enhance contrast. Light microscopy stains are organic & consist of -/+ charged molecules (acidic and basic stains) that bind within the tissue to macromolecules of the opposite charge. Transmission electron microscopy (tem) stains are heavy metal salts that deflect electrons in the beam to different extents, providing contrast to the image. Scanning electorn microscopy (sem) provides 3d pictures of an unsectioned tissue surface. Preserved tissues we see under the microscope were altered and have minor distortions called artifacts. Epithelial tissue: epithelial tissue/epithelium - sheet of cell that covers a body surface/lines a body cavity, covering and lining epithelium, glandular epithelium, function: protection, absorption, filtration, excretion, secretion & sensory reception. Adjacent cells are bound together at many points by lateral contacts, including tight junction and desmosomes. Tight junctions keep proteins in apical region of.
