Biology 2581B Study Guide - Centimorgan, Holliday Junction, Chi-Squared Test

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Recombination, genetic linkage, & the chi square test: Recombination is the sorting of alleles into new combinations and is within the realm of. Mendelian genetics as it occurs during independent assortment. Four combinations results from recombination: 2 are parental (same arrangement of alleles as parents) and 2 are recombinant (carry a novel combination). Genetic linkage is a violation of mendel"s law of independent. Assortment as the resultant gametes deviate from the expected 1:1:1:1 ratio (more parental genotypes observed than recombinant). We can use the chi square test to determine if the deviations from the expected 1:1:1:1 phenotypic ratio are due to sampling error, or if an underlying biological phenomenon is the cause. With the degrees of freedom being 3 (4 classes 1), we conclude that the observed differences from the expected 1:1:1:1 ratio are not due to sampling error (we reject the null hypothesis), but some biological/genetic phenomenon is present.

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