Biology 2290F/G Study Guide - Phospholipid, Streptomycin, Fluorescence
4 Mar 2014
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Document Summary
Because according to lambert"s law, for a thin layer of sample, the same proportion of light is absorbed regardless of the radiant power of the incident light. At the same time, there is a rod that is connected from the grating to a wavelength cam (cam follower) that displays the wavelength emitted as the grating is turned (figure 1). Adjust spectrophotometer for a reference blank. Blank tube is placed in the sample holder and meter adjusted to 100% t (0% a: blank tube should contain all substances in the sample except for the material whose absorbance you wish to measure. To make quantitative determinations of the concentration of materials in solution by making reference to a standard curve. Spectrophotometry can be used to measure an unknown solution concentration if you first prepare a standard curve of known concentrations of that substance vs. absorbance.
