Biochemistry 2280A Lecture Notes - Lecture 5: Reverse Transcriptase, Nitrocellulose, Hydrogen Bond

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The techniques which allow dna fragments from different source to be recombined to make new molecules with unique features. The construction of new biological parts and systems. The redesign of existing, natural systems for useful purposes. Slow: limited by the breeding time of the organism and chance genetic events. Limited to breeding species; must cross between the same species. Rapid: as quick as a few days in some organisms. Definition: the use of organisms to do work for man. Modern biotechnology impacts many aspects of society and relies heavily on recombinant dna technology. Drug production and design rely heavily on recombinant dna technology. Diagnosis of disease - detect pathogens and disease causing genes through their dna. Genetic counselling - does an individual carry a disease related allele. Many diseases result from having a defective gene. We can add back functional genes to cure diseases. These approaches can work, but often putting the gene back can cause other problems.

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