BIOL 1070 Study Guide - Midterm Guide: Mitochondrion, Membrane Fluidity, Mitochondrial Matrix

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16 Mar 2014

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Dna from the environment: eukaryotic (cid:131) proteus, fungi, plants, animals (cid:131) structurally more complex (cid:131) have multiple forms of dna (chromatin -> chromosomes). Dna is enveloped in membrane (nucleus) (cid:131) undergo mitosis, which is the highly regulated production of daughter cells. Membranes possess receptors that combine with specific molecules (ligands) having a complementary structure: intercellular interaction (cid:131) the plasma membrane of multicellular organisms mediates the interactions between a cell and its neighbors. Because most membrane phospholipids are built a=on a glycerol backbone they are called phosphoglycerides. Membrane glycerides are diglycerides (only two of the hydroxyl groups of the glycerol are esterified to fatty acids, the third is esterified to a hydrophilic phosphate group. Membrane phosphoglycerides have an additional group linked to the phosphate, with the group and the phosphate a highly water-soluble head group is formed. Sphingolipids consist of sphingosine linked to a fatty acid by its amino group.

Related Questions

________30. If you are investigating the inner structure of a new cell and see that it has a significantly larger amount of RER than other cells. You would conclude the cell does a great deal of____?
A. ATP synthesis

B. protein synthesis
C. DNA synthesis
D. active transport
E. polysaccharide hydrolysis

________31. If a cell comes in contact with a toxic substance such as H2O2, it could survive as long as it has a ___ to digest it.
A. DNA
B. peroxisome

C. mitochondria
D. phospholipids
E. intermediate filaments

________32. Some cells have structures to help them withstand osmotic pressure. For example,
A. Plant cells due to their walls
B. Animal cells due to the sodium/potassium pumps

C. Bacterial cell due to their swimming

D. Viruses due to their protein coats

________33. Evolution occurs only as a cell ____.

A. They undergo mitosis
B. Change in their gene pool
C. They move from one habitat to another

D. They begin to eat different foods

________34. Ribosomes are produced in the

A. nucleoli.
B. chromatin.
C. nuclear envelopes.

D. Golgi complex. E. cisternae.

________35. Algae are often green, but there are red and brown species also. What accounts for this difference in color?
A. The chlorophyll of chloroplasts
B. The pigments in other plastids

C. The presence of a mitochondria
D. The accumulation of cellular wastes

________36. The chloroplast and other like prokaryotes have which of the following that helps with photosynthesis?
A. They live in water based environments
B. They have chloroplasts in their cytoplasm

C. Change color in the dark vs. the sun
D. They have photoreceptor proteins in their membranes or mesosomes.

________37. Located in the membrane, we see a set of proteins called receptors that only connect with or bind to specific substances. This is called __.
A. ligand specificity.
B. hormone selectivity.

C. cell tonicity.
D. water saturation. E. signal resolution.

________38. Of the following statements, we could say that ___ is false.
A. All organisms are composed of cells and cell products.
B. An organism's structure and all of its functions are due to the activities of cells.
C. The cellular organelles are the simplest structural and functional unit of life.
D. Cells come from preexisting cells.
E. Cells are made from nonliving components including the nucleus, cell membrane and cytoplasm.

________39. If it is really true that our cells are the smallest anatomical unit that meets all of the properties of life, then which of the following could imply otherwise?
A. vacuoles
B. centrioles

C. Golgi apparatus
D. mitochondria
E. endoplasmic reticulum

________40. Oxygen is an absolute need for animal and plant cells alike due to the ___.

A. RER
B. Mitochondria
C. Lysosomes

D. DNA

________41. In a plasma membrane, we expect to see the carb/protein structures to extend into the ___, but the secondary messengers to move within the __.
A. extracellular fluid; cytosol
B. cytosol; extracellular fluid

C. intracellular fluid; cytosol

D. cytosol; intracellular fluid

E. extracellular fluid; plasma

________42. Bacteria are found in all of the following habitats:

A. Caves
B. Oceans
C. Animals

D. Everywhere on Earth

________43. Two solutions are separated by a selectively permeable membrane. Solution A has a higher concentration of an impermeable solute compared to solution B. Which of the following do you expect would happen?
A. solute will move from solution A to solution B

B. solute will move from solution B to solution A

C. water will more from solution A to solution B

D. water will move from solution B to solution A E. no movement of solute or water will occur

________44. In the kidney, we notice that almost all of the cells involved in the production of urine have high concentrations of the Na+/K+ pump. Which typically works to push Na+ ___ its concentration gradient by using ___.
A. up; active transport

B. up; facilitated transport
C. up; cotransport
D. down; active transport
E. down; facilitated transport

________45. In the charts of a patient recently admitted to the hospital noted that he was taking a medication called lithium and high doses of calcium supplements. Given this, we would expect the patientâs liver to have a higher than normal amount of __ to purify out the lithium and calcium.

A. lysosomes.
B. centrosomes.
C. mitochondria.
D. peroxisomes.
E. rough endoplasmic reticulum.

________46. We have long observed that our neural cells in the brain cannot replicate or replace themselves due to the lack of ____.
A. produce ATP.
B. divide.

C. produce enzymes.
D. secrete material across their plasma membrane E. conduct electrical signals.

________47. An organismâs structure and function is ultimately due to the structure and function of itâs __.
A. Organs
B. Tissues

C. Cells

D. Nuclei

Chapter 6: How Cells Harvest Chemical Energy

What is the difference between photosynthesis and cellular respiration?

How do cells capture the energy released by cellular respiration?

What are the products of cellular respiration?

What does pyruvate do?

The conversion of CO2 and H2O into organic compounds using energy from light is called what?

If ATP accumulates in a cell, what happens?

A drug is tested in the laboratory and is found to create holes in both mitochondrial membranes. Scientists suspect that the drug will be harmful to human cells because the drug inhibits what?

As a result of glycolysis, there is a net gain of ________ ATP(s).

Which of the following is a result of glycolysis?

The enzymes of the citric acid cycle are located in what?

Chapter 7 Photosynthesis: Using Light to Make Food

What is the name given to organisms that can make their own food and thus sustain themselves without consuming organic molecules derived from other organisms?

What is an example of a photoautotroph?

What is the origin of chloroplasts?

In the chloroplast, sugars are made in a compartment that is filled with a thick fluid called what?

The oxygen released into the air as a product of photosynthesis comes from what?

What is the use and production of CO₂ in both photosynthesis and cellular respiration?

Know the parts of the chloroplast. Which structure in the chloroplast represents the thylakoid membrane?

What molecules are reactants of photosynthesis?

What is the source of energy that provides the boost for electrons during photosynthesis?

Why are most plants green?

Chapter 8: The Cellular Basis of Reproduction and Inheritance

What is called the creation of genetically identical offspring by a single parent, without the participation of sperm and egg?

How do eukaryotic chromosomes differ from prokaryotic chromosomes?

Prior to mitosis, each chromosome of a eukaryotic cell consists of a pair of identical structures are called what?

Eukaryotic cells spend most of their cell cycle in which phase?

What occurs during interphase?

What is the process called by which the cytoplasm of a eukaryotic cell divides to produce two cells?

What is the phase of mitosis during which the mitotic spindle begins to form?

During which phase of mitosis does the nuclear envelope re-form?

What feature of plant cell division distinguishes it from animal cell division?

What features likely accounts for the difference between plant and animal cell cytokinesis?

What must occur for a plant or animal to grow and develop normally?

Chapter 9: Patterns of Inheritance

Most genetic disorders of humans are caused by what type of alleles?

What is the difference between genotypes and phenotypes?

What are the individual features of all organisms?

What does the chromosome theory of inheritance state?

What is the normal complement of sex chromosomes in a human male?

The sex chromosome complement of a normal human female is what?

How many sex chromosomes are in a human gamete?

What is the gene located on a sex chromosome called?

Why are sex-linked conditions more common in men than in women?

Chapter 12: DNA Technology and Genomics

How long has Biotechnology been around?

What do restriction enzymes do?

What are "Sticky ends"?

What is the best definition of a genomic library?

What is a nucleic acid probe?

The only cells that can correctly attach sugars to proteins to form glycoprotein products are what?

How does a vaccine work?

What is the preferred name of the technique used to determine if DNA comes from a particular individual?

The polymerase chain reaction relies upon unusual, heat-resistant ________ that were isolated from bacteria living in hot springs.

Gel electrophoresis sorts DNA molecules on the basis of their what?

Question 1

1 pts

Cystic fibrosis is caused by nonsense and missense mutations in the CFTR gene, which encodes for a chloride channel. You are studying cystic fibrosis patients to determine what mutation they possess in the CFTR gene. The difference between the mutant and wild type CFTR genes can be uncovered by examining the CFTR:

	DNA
	mRNA
	protein
	tRNA

Question 2

1 pts

You decide to identify the CFTR mutation by analyzing the genomic DNA of your patients compared to healthy individuals. You specifically are looking to see whether a specific 3' gene truncation has occurred in the patients. You will determine this using hybridization techniques with samples from healthy and CF patients. Which of the following will allow you to accomplish this?

	Using an RNA probe complementary to the region not removed by the truncation.
	Using an RNA probe complementary to the region removed by the truncation.
	Using an DNA probe complementary to the region not removed by the truncation.
	Using an DNA probe complementary to the region removed by the truncation.

Question 3

1 pts

You would like to ensure that this experiment (to determine whether patients have a specific CFTR gene truncation using hybridization) is properly controlled. Which of the following samples must you test?

	The genomic DNA of a healthy individual who does not have cystic fibrosis.
	The genome of a CFTR patient known to have the specific truncation you are trying to identify.
	The genome of a CFTR patient with a missense mutation but full length gene.
	The genome of a healthy individual married to a CFTR patient with the specific truncation you are trying to identify.
	The genome of a patient with muscular dystrophy, which can be due to a trucation in the dystrophin gene.

Question 4

1 pts

To conduct the hybridization experiment, you are trying to decide between using a DNA or RNA probe. Which would be ideal to use and why?

	As both are composed of nucleic acids, using either would result in identical results.
	An RNA probe because RNA has uracil bases.
	An RNA probe because it could also be used in a translation experiment.
	A DNA probe because it is more stable than RNA.
	A DNA probe because RNA cannot bind to DNA.

Question 5

1 pts

Which of the following will lower the Tm of a given DNA strand?

	Increasing the percentage of GC base pairs.
	Raising the pH of the solution from neutral to basic.
	Decreasing the buffer concentration from 50mM NaCl to 5mM NaCl.
	None of the above.

Question 6

1 pts

One step of the Hershey/Chase experiment involved blending the virus/cell mixture before centrifugation and probing the pellet for radioactivity. Why was the blending step necessary?

	To collect the bacteria at the bottom of the tube.
	To break open the bacteria to release the genome.
	To separate the bacteria from the bacteriophages.
	To be able to detect the radioactivity.

Question 7

1 pts

Imagine Hershey/Chase had used an RNA virus (genome composed of RNA) instead of a DNA virus in their experiment. Would radioactivity still have been found in the pellet?

	No, because only DNA can be labeled with radioactivity.
	No, because the RNA genome would not enter the bacteria upon infection.
	No, because while DNA and RNA nucleotides are similar, they are not identical.
	Yes, because DNA and RNA nucleotides are similar.
	Yes, because genome in any form (DNA, RNA, protein) would be labeled similarly.

Question 8

1 pts

Griffith and Avery's transformation experiments allowed us to identify that DNA is our genetic information. Which of the following scenarios would result in bacterial cells that are capable of killing mice upon injection?

	Heat killed non-virulent bacteria is added to a live virulent bacteria strain.
	Heat killed virulent bacteria is added to a heat killed non-virulent bacteria strain.
	A heat killed virulent bacteria that is treated with a nuclease, is then added to a non-virulent bacteria strain.
	Heat killed mouse cells are added to a non-virulent bacteria.

Question 9

1 pts

The human genome consists mostly of non-coding DNA. Which of the following are benefits of this?

	Random DNA mutations generally won't affect RNA and protein function.
	It is faster to duplicate the genome when these are present.
	The existence of introns can lead to multiple variations of proteins encoded by a single gene.
	It is unlikely transposons would exist in the genome if there was too much protein coding DNA.

Question 10

1 pts

Andrew Murray's sister, Andrea, is adding to her brother's work on chromosomes. She is using cells that are unable to synthesize adenine (âade) and histidine (âhis). The plasmid she is currently working with consists of an origin of replication and the Ade gene.

Following her transformation of the plasmid into her yeast, what media will the cells be plated on to select for cells that have picked up the plasmid?

	Media containing histidine
	Media containing adenine
	Media lacking adenine
	Media lacking histidine

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BIOL 1070 Study Guide - Midterm Guide: Mitochondrion, Membrane Fluidity, Mitochondrial Matrix

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