BIO SCI D103 Lecture Notes - Phosphoinositide 3-Kinase, Sister Chromatids, Kinetochore
18 Mar 2014
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Document Summary
Microtubule spindle forms to separate chromosomes i. ii. iii. Centrosomes: duplicate during interphase, mother centriole is older than the daughter. Cohesins: mediates cohesions between sister chromatids, phosphorylation on cohesins on chromosome arms causes dissociation. Cohesins are only present on the centromere area: requires atp for chromosome condensation. Possible without condensins, but chromosomes become fragile: phosphorylation. Microtubule dynamic instability: stathmin i. i. i. Office hour: kinesin 13, xmap215, jak kinase i. i. i. ii. Reduces the local concentration of free dimeric tubulin. Decrease the stability: elongates twice as fast during mitosis. Interphase mt - can recover b: mitosis mt - cant recover. Xmap215: stabilizing map, phosphorylated by m-cdk before mitosis. Plus end is near the zone of inter-digitation. Minus ends are near the spindle pole i. ii. ii. ii. Moves them to the plus end of the kinetochore mt. Mediates disassembly at the + end of the kinetochore mt. Inactivation leads to anaphase b: dynein i. ii. iii.
